Oryzalin-induced polyploidy in Borago officinalis reveals cell-wall remodelling via immunofluorescence microscopy
Year: 2025
Type: article
Abstract: Introduction: Artificial polyploidisation is a powerful biotechnological approach for improving morphological and physiological traits in medicinal plants. We investigated the consequences of chemically induced whole-genome duplication in Borago officinalis L. Methods: Tetraploidy was induced in vitro using oryzalin. Flow cytometry verified the establishment of mixoploid and stable tetraploid subclones. Selected tetraploids were evaluated for morphology, anatomy, and cellular features using light/confocal microscopy and immunofluorescence labelling of pectic epitopes (homogalacturonan and rhamnogalacturonan I). Results: Relative to diploids, tetraploids displayed thicker, darker green leaves, increased trichome density, and a distinct growth habit. Microscopy showed significantly enlarged stomata with reduced density, expanded vascular tissues, and altered mesophyll organisation. Immunofluorescence revealed distinct patterns of cell-wall remodelling in tetraploid tissues. Discussion: These findings illuminate the structural and histochemical consequences of genome-dosage changes in B. officinalis and highlight the potential of chemically induced polyploidy to enhance agronomic and pharmaceutical traits. The work provides a platform for future applications in plant metabolic engineering and molecular pharming.
Source: Frontiers in Plant Science
Institutions: Palacký University Olomouc, University of Silesia in Katowice
Language: English
Subfield: Plant Science
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $3,801
OpenAlex ID: W4415836788