Work

NRF2 Activation Suppressed Methylglyoxal-Lysine Dimer-Induced Oxidative Stress and Inflammatory Paracrine Interaction between Macrophages and Adipocytes

Year: 2025

Type: article

Abstract: Macrophage infiltration is closely associated with adipocyte-related inflammatory response in obesity.In this study, we explored methylglyoxal-lysine dimer (MOLD)-induced inflammatory responses and metabolic disorders during macrophage and adipocyte interactions using an in vitro conditioned co-culture medium-based.MOLD significantly increased the levels of advanced glycation end products (AGEs) and receptor for AGEs and induced the levels of nitric oxide and prostaglandin E2 in RAW 264.7 cells.Moreover, MOLD-induced inflammatory cytokines, including tumor necrosis factor alpha and interleukin-6, in RAW 264.7 cells.Macrophage-derived conditioned medium induced adipogenesis transcription factors and lipid accumulation in 3T3-L1 cells.NF-E2 p45-related factor 2 (NRF2) activation suppressed the MOLD-induced expression of inflammation-and adipogenesis-related genes in RAW 264.7 cells.These findings suggest that MOLD stimulates inflammatory response and adipogenesis, and NRF2 may be a key modulator of MOLD-induced inflammation and adipogenesis.

Source: Preventive Nutrition and Food Science

DOI: 10.3746/pnf.2025.30.5.450

Authors: Hee-Weon Lee, Min Ji Gu, Donghwan Kim, Sang Keun Ha

Institutions: Korea Food Research Institute, Daegu-Gyeongbuk Medical Innovation Foundation, Korea University of Science and Technology

Language: English

Topic: Adipokines, Inflammation, and Metabolic Diseases

Subfield: Epidemiology

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: hybrid

Funder: Korea Food Research Institute