Work

R-loop accumulation promotes OSCC malignant progression by upregulating MARVELD3 expression

Year: 2025

Type: article

Abstract: OBJECTIVE: The R-loop is known to play a critical role in the progression of various cancers, yet its involvement in OSCC remains unexplored. This study aims to investigate the role and mechanism of R-loop-mediated MARVELD3 in OSCC. METHODS: DRIP-seq was employed to assess the involvement of the R-loop in OSCC progression. RT-PCR, IHC, and immunofluorescence were utilized to measure MARVELD3 expression and the relationship with R-loop. The functional role of MARVELD3 in OSCC was investigated through proliferation assays, colony formation assays, Transwell assays, and flow cytometry. The regulatory mechanism underlying MARVELD3 was further investigated using RNA-seq, western blotting, and quantitative PCR (qPCR). RESULTS: Dysregulation of R-loop-mediated genes holds significant biological relevance in OSCC. MARVELD3 was found to be highly expressed in OSCC cells due to R-loop accumulation; knockdown of MARVELD3 led to reduced proliferation, migration, and invasion, while MARVELD3 overexpression enhanced these characteristics. Mechanistically, MARVELD3 promotes the MAPK signaling pathway by suppressing the expression of TTC9. CONCLUSION: In conclusion, R-loop accumulation promotes transcription of the MARVELD3. MARVELD3 exerts a tumor-promoting effect in OSCC by downregulating TTC9 and activating the MAPK signaling pathway.

Source: Bioorganic Chemistry

DOI: 10.1016/j.bioorg.2025.109180

Authors: Xinyi Li, Xuerong Xiang, Jiao Huang, Tingxiu Xiang, Ping Ji

Institutions: Stomatological Hospital of Chongqing Medical University, Chongqing University, Chongqing Cancer Hospital

Language: English

Topic: Cancer-related molecular mechanisms research

Subfield: Cancer Research

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: hybrid

Estimated APC paid: $4,240