Work

Simple Method for Sucrose Determination Based on Self-Assembled Nano-Levan Synthesized by Levansucrase

Year: 2025

Type: article

Abstract: High Resolution Image Download MS PowerPoint Slide A simple and specific method for sucrose quantification was developed using nanolevan formation catalyzed by levansucrase from Erwinia tasmaniensis ( Et Lsc). When Et Lsc reacted with sucrose, it quickly produced nanolevan, which generated turbidity that could be measured using spectrophotometry. This method showed a strong linear correlation between optical density and sucrose concentration within the range of 0.2–3.0% w/v. Optimal conditions for the reaction were determined to be pH 6.0–8.0 and enzyme concentration of 20 U/mL. The method demonstrated high specificity toward sucrose without interference from other common sugars. Additionally, a smartphone-based detection approach was developed and validated, providing a portable alternative for on-site analysis. When applied to real fruit juice samples, the results were less than 9% different from those of HPLC analysis. This approach offers a cost-effective, rapid, and user-friendly alternative for sucrose determination in food and beverage samples.

Source: ACS Omega

DOI: 10.1021/acsomega.5c06620

Authors: Thanapon Charoenwongpaiboon, Karan Wangpaiboon, Rath Pichyangkura, Kamontip Kuttiyawong

Institutions: Silpakorn University, Chulalongkorn University, Kasetsart University

Language: English

Topic: Enzyme Catalysis and Immobilization

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Zero hunger

Open Access status: gold

Estimated APC paid: $1,935