Work

Toxic Tear Gas 2‐Chloroacetophenone (CN) Forms Adducts With Endogenous Plasma Thiols In Vitro Valuable as Biomarkers of Exposure

Year: 2025

Type: article

Abstract: As the tear gas of the highest toxicity, 2-chloroacetophenone (CN) poses a potential threat for exposed individuals. Several fatality cases following exposure to CN are documented, but an unambiguous identification of CN exposure is still missing. Thus, we herein present the identification of in vitro reaction products between CN and endogenous molecules useful as biomarkers. After incubation of human plasma with CN, diverse acetophenone (AcPhen)-adducts were formed with the small molecule thiols homocysteine (HCys), glutathione (GSH), and cystine (Cys-Cys). All adducts were detected by microbore liquid chromatography-electrospray ionization high-resolution tandem mass spectrometry (μLC-ESI MS/HRMS) working in the parallel reaction monitoring (PRM) mode and were characterized as potential biomarkers of CN exposure. Time- and concentration-dependent adduct formations were investigated, and the stability of AcPhen-adducts in plasma during 4 freeze-and-thaw cycles and in the autosampler was tested. The limit of identification (LOI) of identified AcPhen-adducts in vitro was found at about 6 μM (concentration of CN in plasma) but showed quite limited in vitro stability. The AcPhen-adducts herein presented might be beneficial for future studies addressing CN exposure in vivo.

Source: Drug Testing and Analysis

DOI: 10.1002/dta.3964

Authors: Paula Helena Sieber, Dirk Steinritz, Franz Worek, Harald John

Institutions: Institut für Pharmakologie und Toxikologie der Bundeswehr, Walther-Straub-Institut für Pharmakologie und Toxikologie, Ludwig-Maximilians-Universität München

Language: English

Topic: Forensic Toxicology and Drug Analysis

Subfield: Toxicology

Field: Pharmacology, Toxicology and Pharmaceutics

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: hybrid

Estimated APC paid: $4,440