Mechanistic insights into the Bacillus subtilis K6 solid-state fermentation-induced structural transformation and enhanced bioactivity of flaxseed meal dietary fiber
Year: 2025
Type: article
Abstract: K6. This study systematically examined structural modifications and in vitro bioactivities of soluble (SDF) and insoluble dietary fiber (IDF) fractions before and after fermentation. SEM and FTIR analyses showed degradation of cellulose and hemicellulose during SSF, yielding a more porous structure. XRD and TGA indicated disrupted crystalline regions in SDF and IDF, with fermented IDF (F-IDF) exhibiting enhanced thermal stability. SSF altered monosaccharide composition and reduced DF molecular weight. Transcriptomics revealed pentose and glucuronate interconversions and starch and sucrose metabolism as primary DF dissociation pathways, dominantly mediated by extracellular hydrolases. In vitro assessments demonstrated F-IDF's superior glucose adsorption capacity, whereas fermented SDF (F-SDF) exhibited greater cholesterol adsorption, pancreatic lipase inhibitory activity, nitrite adsorption capacity, and sodium cholate binding capacity. These findings provide a theoretical basis for FSM-derived DF utilization.
Source: Food Chemistry X
Authors: Chunpeng Hou, Jiaxun Chen, Chenxian Yang, Xiaohui Geng, Ying Xin, Fusheng Chen
Institution: Henan University of Technology
Language: English
Subfield: Nutrition and Dietetics
Field: Nursing
Domain: Health Sciences
SDG: Zero hunger
Open Access status: gold
Estimated APC paid: $2,330
Funders: National Natural Science Foundation of China, Henan University of Technology, Hunan Association for Science and Technology, National Key Research and Development Program of China, National Major Science and Technology Projects of China, Henan Provincial Science and Technology Research Project
OpenAlex ID: W4415900780