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CIAPIN1 as a Promoter of Oral Squamous Cell Carcinoma Progression

Year: 2025

Type: article

Abstract: OBJECTIVE: This study aims to investigate the relationship between the expression of cytokine-induced apoptosis inhibitor 1 (CIAPIN1) and the development of oral squamous cell carcinoma (OSCC). MATERIALS AND METHODS: Bioinformatics analyzed CIAPIN1 in pan-cancers; IHC detected CIAPIN1 expression in OSCC and healthy samples; qRT-PCR and WB assayed CIAPIN1 mRNA and protein levels in OSCC and normal cells; MTT, scratch, transwell, flow cytometry, and WB evaluated OSCC cell viability, migration, invasion, EMT, and apoptosis after CIAPIN1 knockdown. RESULTS: CIAPIN1 abnormally expressed in multiple tumors, especially OSCC, is linked to prognosis. IHC showed elevated CIAPIN1 in OSCC tissues (p < 0.001). qRT-PCR and WB results showed CIAPIN1 was upregulated in CAL-27 (qRT-PCR: p < 0.05, WB: p < 0.05), SAS (qRT-PCR: p < 0.05, WB: p < 0.01), and SCC9 (qRT-PCR: p < 0.01, WB: p < 0.001) cells compared to HOK cells. After the knockdown of CIAPIN1 in SAS and SCC9 cells, MTT assays revealed reduced cell viability (SAS: p < 0.001, SCC9: p < 0.001); scratch tests showed suppressed migration ability (SAS: p < 0.01, SCC9: p < 0.001); transwell assays indicated inhibited invasion capability (SAS: p < 0.01, SCC9: p < 0.001); flow cytometry revealed increased apoptosis rates (SAS: p < 0.01, SCC9: p < 0.01); WB analysis showed reduced N-cadherin expression and increased E-cadherin expression (SAS for N-cadherin: p < 0.05, SCC9 for N-cadherin: p < 0.01; SAS for E-cadherin: p < 0.01, SCC9 for E-cadherin: p < 0.05), indicating CIAPIN1 promotes EMT. CONCLUSION: CIAPIN1 contributes to the malignant progression of oral squamous cell carcinoma.

Source: Journal of Oral Pathology and Medicine

DOI: 10.1111/jop.70083