Work

Valorization of Caragana korshinskii Kom. using cooperative Aspergillus oryzae and Saccharomyces cerevisiae to produce fermented feed protein

Year: 2025

Type: article

Abstract: Caragana korshinskii Kom. represents a substantial biomass resource that can be converted into feed protein via microbial fermentation. This study aimed to improve the nutritional value of C. korshinskii through strain screening and substrate optimization. Amino acid content and in vitro digestibility were systematically investigated. Astral-DIA proteomics was employed to compare protein enrichment mechanisms underlying screened microbial involvement in substance conversion. The Aspergillus oryzae and Saccharomyces cerevisiae co-culture increased the true protein content of the optimized substrate by 50.6% to 67.9%, while the highest nitrogen conversion ratio (69.5%) was achieved with low-level supplementation of (NH 4 ) 2 SO 4 . The relative abundances of hydroxyproline and lysine content increased by more than twice in the mixed fermentation. Proteomics analysis identified 291 differentially expressed proteins in the mixed culture versus A. oryzae alone, enriched in ribosome biogenesis; valine, leucine and isoleucine biosynthesis; galactose metabolism; amino acids biosynthesis and sulfur relay system. This study provided guidance for the high-value utilization of C. korshinskii and elucidated the differential protein enrichment pathways between A. oryzae , S. cerevisiae and their cocktail in utilizing C. korshinskii . Graphical abstract

Source: Bioresources and Bioprocessing

DOI: 10.1186/s40643-025-00968-4

Authors: Sasa Zuo, Jing Su, Fuqiang Zhang, Shuying Yu, Xiaohui Cao, Chuncheng Xu

Institution: China Agricultural University

Language: English

Topic: Biofuel production and bioconversion

Subfield: Biomedical Engineering

Field: Engineering

Domain: Physical Sciences

SDGs: Zero hunger, Responsible consumption and production

Open Access status: gold

Estimated APC paid: $1,890