Work

Pulsed amperometric detection: Extending the applicability of HPLC to substances with two-step redox processes

Year: 2025

Type: article

Abstract: Electrochemical detection coupled to liquid chromatography provides high selectivity compared to optical detection due to the lower number of electrochemically active compounds. Nevertheless, not all electrochemically active substances may be detected with the classical amperometric detection that involves the application of a single potential. For instance, in flow conditions, the main biomarkers of cinnamon (eugenol, coumarin, cinnamaldehyde and cinnamic acid) require an oxidation step prior to their amperometric detection. Pulsed amperometric detection (PAD) is proposed to address the electrochemical determination of substances with such complex redox behaviour in flow conditions because it allows the application of multiple potential steps that mimic the sequential stages that would be performed in static voltammetric measurements. PAD waveform has been optimized resulting in two scenarios (t ox >> t red and t ox <

Source: Microchemical Journal

DOI: 10.1016/j.microc.2025.116086

Authors: Josep Pages-Rebull, Clara Pérez-Ràfols, Núria Serrano, José Manuel Díaz-Cruz

Institution: Universitat de Barcelona

Language: English

Topic: Electrochemical Analysis and Applications

Subfield: Electrochemistry

Field: Chemistry

Domain: Physical Sciences

Open Access status: hybrid

Estimated APC paid: $4,000

Funders: Generalitat de Catalunya, European Social Fund