Identification of distinct cDC2 subpopulations that direct microbiota-specific T cell differentiation
Year: 2025
Type: preprint
Abstract: How the complex network of intestinal antigen presenting cells (APCs) instructs CD4 + T cell responses against the microbiota remains unclear. Here, we use Labeling Immune Partnerships by SorTagging Intercellular Contacts (LIPSTIC) to characterize the APCs that prime CD4 + T cells recognizing the commensal bacterium Akkermansia muciniphila . A. muciniphila -specific T cells engaged multiple transcriptionally distinct migratory cDC2 subpopulations, both at homeostasis, when A. muciniphil a promotes T FH differentiation, and during inflammation, when it also drives T H 1 and T H 17 differentiation. The identity of these subpopulations was unchanged by inflammation; however, the distribution of presentation across the subpopulations shifted, with increased presentation by inflammatory cDC2s favoring T H 1 and T H 17 polarization. These results reveal how distinct T cell differentiation trajectories can be determined through varied interactions with multiple, functionally distinct subpopulations of APCs.
Authors: Shaina L. Carroll, Andrew Ly, Ashley K. Liu, Maria C. C. Canesso, Gabriel D. Victora, Daniel Mucida, Gregory M. Barton
Language: English
Topic: T-cell and B-cell Immunology
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: green
Awards: Differential Regulation of T Cell Responses to the Commensal Bacterium Akkermansia Muciniphila, Defining the role of T cell help in germinal centers by intercellular enzymatic labeling, Immunology & Molecular Medicine Program Training Grant, Intestinal CD4 T cell responses to dietary and microbial antigens, Cell-cell interactions driving gut inflammation and tolerance
OpenAlex ID: W4415936197