Year: 2025
Type: article
Abstract: Nicotinic acid adenine dinucleotide phosphate (NAADP) is a second messenger that stimulates intracellular Ca2+ release in both mammalian cells and echinoderm egg homogenates. A NAADP linked covalently to a stable long-wavelength fluorescent dye would be a useful probe with which to characterize NAADP–receptor interactions in solution and potentially to determine intracellular-binding localization. We report the synthesis of a BODIPY-NAADP covalent conjugate made through linking the carboxyl group of BODIPY FL to the primary amino group of 5-(3-aminopropyl)-NAADP through amide bond formation. The starting pyridine dinucleotide analog, 5-(3-aminopropyl)-NAADP was available through enzyme-catalyzed base exchange between NADP and a substituted nicotinic acid analog. The resulting 5-BODIPY-NAADP conjugate was purified to homogeneity using ion-exchange chromatography, was produced in milligram quantities, and its spectroscopic properties were characterized.
Source: Molbank
DOI: 10.3390/m2085
Authors: Zhong Guan, James T. Slama
Institution: University of Toledo
Language: English
Subfield: Spectroscopy
Field: Chemistry
Domain: Physical Sciences
Open Access status: gold
Estimated APC paid: $603
Funder: National Institutes of Health
Award: G9000144862
OpenAlex ID: W4415952764