Simultaneous real-time multispectral fluorescence and reflectance imaging for enhanced intraoperative guidance
Year: 2025
Type: article
Abstract: Intraoperative fluorescence imaging enhances surgical precision, but current systems require switching between anatomical and fluorescence views, disrupting workflow and forcing surgeons to mentally overlay separate images. We developed a dual-sensor multispectral imaging system that uniquely enables simultaneous visualization of full-color anatomy and multiple fluorescence signals (protoporphyrin IX, fluorescein sodium, and indocyanine green) in real-time, a capability not available in existing surgical platforms. By employing custom optical filters and linear unmixing algorithms, the system captures all imaging modes concurrently, providing perfectly co-registered anatomical and fluorescence information in a single unified view. Phantom validation demonstrated accurate fluorophore separation while maintaining color fidelity for tissue visualization. This approach eliminates mode-switching delays and mental image fusion, streamlining surgical workflow with a practical platform designed for clinical integration.
Source: Optics Continuum
Language: English
Subfield: Radiology, Nuclear Medicine and Imaging
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $1,372
OpenAlex ID: W4415953175