Work

Application of catalytic hairpin assembly reaction in liquid biopsy

Year: 2025

Type: article

Abstract: Liquid biopsies enable noninvasive and dynamic analysis of cellular and extracellular vesicle biomarkers. The catalytic hairpin assembly (CHA) provides an enzyme-free isothermal amplification technique characterized by high catalytic efficiency, sensitivity, and specificity. It holds significant potential for non-invasive, cost-effective, and straightforward detection of tumor biomarkers, offering versatile signal output modalities such as fluorescence, electrochemical, and colorimetric methods. CHA signal amplification technique has potential to detect tumor biomarkers with non-invasive, low-cost, highly sensitive and highly specific advantages. Herein, the varied CHA mechanisms, hairpin probe designs, and reaction conditions are introduced in detail. Additionally, biosensors based on CHA for the early detection of cancer biomarkers are presented. Finally, some views on the challenges and prospects of CHA in the field of biosensing are also considered.

Source: Talanta Open

DOI: 10.1016/j.talo.2025.100591

Authors: Zhilong Yao, Mei Wang, Jiezhen Pan, Xiujuan Huang, Jian Zhang, Shihua Luo

Language: English

Topic: Advanced biosensing and bioanalysis techniques

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $2,190

Funders: National Natural Science Foundation of China, China Postdoctoral Science Foundation