Work

The effect of yeast-derived prebiotics on mitigating colonization and fecal shedding of Salmonella and modifying the microbiome of laying hens in a Salmonella Enteritidis challenge

Year: 2025

Type: article

Abstract: This study was conducted to evaluate the effects of two yeast-based prebiotics on mitigating Salmonella Enteritidis (SE) infection and modifying the microbiome of layer chickens. At 45 weeks of age, 72 Hy-line W36, SE negative, layers were placed in a tiered battery of 36 cages in a randomized block design. Tiers of cages were considered as the blocking factor, and each cage housed two layers. A four-week adaptation period was allowed before the oral challenge with nalidixic acid resistant SE (10^9 CFU/bird). A common diet was supplied to birds with or without prebiotic supplementation to formulate four treatments: Unchallenged control (NC), challenged control (PC), PC+ hydrolyzed yeast+yeast culture (HY/YC, 100 g/ton), and PC+ yeast cell wall+hydrolyzed yeast (YCW/HY, 100 g/ton). Fecal samples were collected at 0-, 3-, 7- and 14-days post inoculation (dpi) to quantify SE load in feces. Additionally, one bird from each cage was euthanized on 7- and 14-dpi to determine SE load in liver, ovary, spleen and ceca. Cloacal swabs were collected from one layer per replication, from replications one to six, on 14-dpi for 16S rRNA sequencing and analysis of microbiome richness, diversity, and robustness. Data were log transformed and analyzed using PROCGLIMMIX procedure of SAS 9.4 and means were separated by Fisher’s LSD. At 7-dpi layers fed HY/YC or YCW/HY had a 1 log reduction in fecal shedding and cecal colonization of SE compared to PC (feces: 0.939 and 0.761 Vs 1.657; Ceca 1.594 and 1.597 Vs 2.613 logCFU/gm), without compromising overall egg production. Microbiome richness was better for layers fed NC or HY/YCW than for layers fed PC. Microbiome diversity and robustness were better for layers fed NC or HY/YC than for layers fed PC. Thus, yeast-based prebiotics could be effective in mitigating SE infection, as well as improving the microbiome of SE challenged layers.

Source: The Journal of Applied Poultry Research

DOI: 10.1016/j.japr.2025.100634

Authors: Fozol K. Ovi, Ishab Poudel, Theresia Lavergne, Charlie Elrod, Pratima Adhikari

Institutions: North Carolina State University, Mississippi State University

Language: English

Topic: Animal Nutrition and Physiology

Subfield: Animal Science and Zoology

Field: Agricultural and Biological Sciences

Domain: Life Sciences

SDGs: Zero hunger, Good health and well-being

Open Access status: gold

Estimated APC paid: $2,300