Recent advances in the analysis of unsaturated fatty acids in biological samples based on chemical derivatization and mass spectrometry techniques
Year: 2025
Type: article
Abstract: Fatty acids (FAs) play a vital role in various physiological processes in the human body, and the analysis of FA isomers is of utmost importance in the research of various diseases and FA metabolic pathways. Over the last few years, the use of chemical derivatization methods to alter the composition of FAs has been found to be highly effective in overcoming the drawback of poor sensitivity in mass spectrometry analysis due to the poor ionizability of functional groups in FA molecules. Furthermore, certain chemical derivatization methods have enabled detection of C=C and differentiation of cis-trans isomers. As a result, the development and use of chemical derivatization-based mass spectrometry to analyze FAs has gained significant interest. This review highlights technological innovations in the development of mass spectrometry detection methods for unsaturated FAs (UFAs) based on chemical derivatization. Special emphasis is placed on two significant strategies: carboxyl group derivatization and C=C derivatization. In addition, it focuses on applications in various fields, discusses persistent challenges, and explores potential future directions. This review aims to provide a better perspective on the advanced design and development of mass spectrometry detection methods of FAs based on chemical derivatization. • Derivatization based on fatty acid carboxyl groups has greatly improved the sensitivity of fatty acids in mass spectrometry analysis. • Derivatization techniques targeting carbon-carbon double bond provide effective tools for precise analysis of the fatty acid positional isomers. • Comprehensive characterization of fatty acids improve the response sensitivity of fatty acids in MS analysis while accurately pinpoint the position of double bonds within fatty acids.
Authors: Yuanyuan Lin, Ningbo Chen, Wenda Chen, Fan Yin, Ling Yang, Xuan Chen, Jian-Liang Zhou, Tian Xie
Institution: Hangzhou Normal University
Language: English
Subfield: Spectroscopy
Field: Chemistry
Domain: Physical Sciences
Open Access status: gold
Estimated APC paid: $1,950
OpenAlex ID: W4415973334