Work

Dissecting Heterogeneity Reveals Functional Subpopulation of Stem Cells From Human Exfoliated Deciduous Teeth

Year: 2025

Type: article

Abstract: OBJECTIVES: Identify the functional subpopulation's surface marker of stem cells from human exfoliated deciduous teeth (SHEDs) using single-cell RNA sequencing (scRNA-seq) and isolate the subset, providing a theoretical basis for the refined application of stem cells. METHODS: subpopulation was also verified through cratch tests, transwell migration assays, qPCR, Matrigel tube formation assays, and chick embryo chorioallantoic membrane (CAM) assay. RESULTS: Major subpopulations of SHEDs, including a proliferative subpopulation, a functional subpopulation, fibroblast-like cells, were identified. As for the transformation trajectories, the proliferative subpopulation is the root population, which then differentiates into a functional subpopulation or fibroblast-like cells. The functional subpopulation is characterized by BAMBI expression and exhibits strong angiogenic, chondrogenic, and proliferative capabilities. Cells at different passages presented distinct and common characteristics. CONCLUSIONS: SHEDs constitute a heterogeneous population and BAMBI⁺ subpopulation has higher proliferative, chondrogenic and angiogenic capacities. CLINICAL RELEVANCE: This study offers a theoretical basis for optimizing SHED-based cell therapeutic strategies. BAMBI⁺ SHEDs, excelling in angiogenesis/chondrogenesis, may be used for tissue-engineered therapies of the clinical disorders such as temporomandibular joint disc regeneration via "vascularize-then-cartilage" strategy.

Source: International Dental Journal

DOI: 10.1016/j.identj.2025.104014