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Identification of byakangelicin metabolites in rats via the UHPLC-Q-exactive orbitrap mass spectrometer

Year: 2025
Type: article
Abstract: Byakangelicin is a furanocoumarin derived from the roots of Angelica dahurica. It exhibits pharmacological properties, including anti-tumor activity, as well as against liver injury and fibrosis. In this study, Ultra-High-Performance Liquid Chromatography with Q-Exactive Orbitrap Mass Spectrometry technology was employed to investigate the metabolic profile of byakangelicin in rats. Following intragastric administration the suspension of byakangelicin, plasma and tissue specimens were harvested. According to high-resolution extracted ion chromatograms, the metabolites of byakangelicin were identified by comparing accurate mass, diagnostic fragment ions, and chromatographic retention times. Data collection was performed in positive ion mode to facilitate metabolite characterization of byakangelicin. Overall, 46 metabolites were successfully characterised . The primary metabolic pathways included hydroxylation, dehydroxylation, methylation, demethylation, reduction, glucuronidation, glycination, and cysteinylation. This systematic investigation of the metabolic characteristics and pathways of byakangelicin in rats provides a valuable reference for future pharmacodynamic evaluations, pharmacological research, and drug development. • For the first time, 46 compounds of byakangelicin were identified using UHPLC-Q-Exactive Orbitrap MS. • In vivo reactions such as hydroxylation, methylation, reduction, glucuronidation, glycination, and cysteinylation, and complex combinations of these were investigated. • To explore the cleavage mode and tissue distribution characteristics of the metabolism of byakangelicin in rats.
DOI: 10.1016/j.jchromb.2025.124841
Language: English
Field: Medicine
Open Access status: hybrid
Estimated APC paid: $3,640
OpenAlex ID: W4416020453
FWCI:
Cites: 24
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