Scanning probe microscopy for rheological analysis of biomolecular condensates
Year: 2025
Type: article
Abstract: Biomolecular condensates are membrane-less phase-separated assemblies that play key roles in cellular organization. Here, we present a protocol to characterize the full range of rheological properties of condensate droplets, from liquid to solid states, using scanning probe microscopy. We describe steps for preparing condensates, cantilever calibration, and condensate measurement. We then detail analysis procedures required to derive the complex shear modulus. For complete details on the use and execution of this protocol, please refer to Naghilou et al. 1 • Protocol for measuring condensate rheology via scanning probe microscopy • Steps for condensate preparation, cantilever calibration, and rheology analysis • Data acquisition and analysis for viscoelasticity from force-distance curves Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. Biomolecular condensates are membrane-less phase-separated assemblies that play key roles in cellular organization. Here, we present a protocol to characterize the full range of rheological properties of condensates, from liquid to solid states, using scanning probe microscopy. We describe steps for preparing condensates, cantilever calibration, and condensate measurement. We then detail analysis procedures required to derive the complex shear modulus.
Source: STAR Protocols
Authors: Aida Naghilou, Alireza Mashaghi
Institutions: Leiden University, Medical University of Vienna
Language: English
Subfield: Atomic and Molecular Physics, and Optics
Field: Physics and Astronomy
Domain: Physical Sciences
Open Access status: gold
Estimated APC paid: $1,550
OpenAlex ID: W4416024195