Diagnostic Potential of Circulating lncRNA SNHG5 in Osteoporotic Fractures and Its Mechanistic Role in Accelerating Fracture Healing
Year: 2025
Type: article
Abstract: OBJECTIVE: This study aimed to investigate the diagnostic value of lncRNA SNHG5 in osteoporotic fractures (OPF) and its molecular mechanism in promoting fracture healing. METHODS: A total of 120 patients with OPF and 126 patients with OP were included in this study. The expression levels of SNHG5, miR-23a-3p, RUNX2, ALP, OCN, and OPN were detected by RT-qPCR. The diagnostic value of SNHG5 was assessed by ROC curve analysis. CCK-8 assay and flow cytometry were used to detect cell proliferation and apoptosis. The targeting relationship between SNHG5 and miR-23a-3p was predicted by bioinformatics and verified by dual luciferase reporter assay. RESULTS: SNHG5 was significantly downregulated in OPF patients, particularly in those with delayed healing. Elevated SNHG5 downregulated miR-23a-3p and concurrently increased the expression of osteogenic differentiation-related genes (RUNX2, ALP, OCN, and OPN) in MC3T3-E1 cells. In addition, SNHG5 promotes osteoblast proliferation and inhibits apoptosis. Mechanistically, SNHG5 is directly targeted by miR-23a-3p, which inhibits SNHG5-mediated effects on osteoblasts. CONCLUSION: SNHG5 indirectly enhances osteoblast activity by negatively regulating miR-23a-3p, thereby promoting fracture healing. Our study could furnish both theoretical and experimental support for the discovery of innovative treatment targets.
Source: Journal of Investigative Surgery
Authors: Changqing Shao, Jin Li, Jiaojiao Chen, Liang Han, Xiaowei Jiang, Lei Shang
Institutions: Xuzhou Central Hospital, Xuzhou Rehabilitation Hospital
Language: English
Topic: Bone Metabolism and Diseases
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $2,970
OpenAlex ID: W4416066298