Work

Design and optimization of caspase-1-responsive fluorescent probes for pyroptosis imaging and anti-pyroptosis drug screening

Year: 2025

Type: article

Abstract: intervertebral disc degeneration and osteoarthritis. Moreover, we combined FPy1 with high-content analysis to establish a screening platform for caspase-1 modulators, based on the classic NLRP3 inflammasome-mediated caspase-1 activation model in primary macrophages. Collectively, these results illustrated the potential of FPy1 as a versatile tool for tracking the progression of pyroptosis and monitoring caspase-1 activity across various application scenarios.

Source: Chemical Science

DOI: 10.1039/d5sc07690k

Authors: Wei Wang, Wei Wang, Guanrui Huang, Yeting Zhou, Yue Wang, Yue Wang, Luling Wu, Tony D. James, Weili Wang, Weili Wang, Yi Wang, Yi Wang

Institutions: Second Affiliated Hospital of Zhejiang University, Zhejiang University, First Affiliated Hospital Zhejiang University, State Key Laboratory of Analytical Chemistry for Life Science, University of Bath, Nanjing University, Henan Normal University

Language: English

Topic: Inflammasome and immune disorders

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: diamond

Funders: Henan Normal University, University of Bath, National Natural Science Foundation of China, National Key Research and Development Program of China, Fundamental Research Funds for the Central Universities, Natural Science Foundation of Zhejiang Province

Awards: G1120405576, G1182693096, G2895029101, G4471023547, 巨噬细胞在冠状病毒-宿主互作中的多器官免疫稳态调控效应及分子机制研究, G6169295534, G8415433584

OpenAlex ID: W4416067667

FWCI: 1.263

Cites: 41

Cited by: 5

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