The Role of Glyceraldehyde-3-Phosphate Dehydrogenase in 2-Ketogluconic Acid Industrial Production Strain Pseudomonas plecoglossicida JUIM01
Year: 2025
Type: article
Abstract: The full-length gapA gene (1002 bp) was cloned from Pseudomonas plecoglossicida JUIM01, an industrial strain used for 2-ketogluconic acid (2KGA) production. The protein encoded by gapA (designated Gap) was predicted to be a canonical NAD(P)-dependent glyceraldehyde-3-phosphate dehydrogenase that catalyzes the interconversion between glyceraldehyde-3-phosphate and 1,3-bisphosphoglycerate. Bioinformatics analyses and electrophoretic mobility shift assays suggested that gapA is regulated by the transcription factor HexR. Through the knockout and complementation of the gene, along with shake-flask experiments and fermentation in bioreactors, this study demonstrated that the deletion of gapA increased the 2KGA production, sugar–acid conversion rate, molar yield, and productivity of P. plecoglossicida JUIM01 by 5.7–6.6% without affecting cell growth, highlighting the mutant’s significant industrial potential.
Source: Foods
Authors: Lei Sun, Lei Sun, Dao-Jiao Tang, Qian-Nan Zhang, Lu-Lu Li, Lei Zhang, Xin-Yi Zan, Feng-Jie Cui, Ling Sun, Ling Sun, Wen-Jing Sun
Institution: Jiangsu University
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $3,499
Awards: G2485879536, G5135892732, G6694215616
OpenAlex ID: W4416069620