An ic-ELISA with carboxymethyl chitosan immunomagnetic beads purification process for the determination of T-2 toxin in cereals
Year: 2025
Type: article
Abstract: T-2 toxin is a common secondary metabolite of the Fusarium fungi. It is toxic and widely distributed in field crops and during the storage of grains. To detect it, in this study, we developed an indirect competition enzyme-linked immunosorbent assay (ic-ELISA) with Fe 3 O 4 -carboxymethyl chitosan-antibody (Fe 3 O 4 -CMCS-Ab) nanoparticles-based sample purification method. Fe 3 O 4 -CMCS-Ab nanoparticles could eliminate the matrix effect and concentrate T-2 toxin to improve the detection limit and detection capability. The detection limit (LOD) and linear range of the ic-ELISA method with Fe 3 O 4 -CMCS-Ab sample purification were 0.03 ± 0.01 µg/L and 0.07 ± 0.01–1.20 ± 0.02 µg/L, respectively. The assay was validated using wheat, oat, and corn samples. The LODs in wheat, oat, and corn samples were 19.12 ± 0.86, 17.88 ± 0.35, and 20.48 ± 0.57 µg/kg, respectively.
Authors: Juwei Ba, Fuyuan Bian, Yafei Zhang, Wentao Liu, Mingxin Li, Lingjie Sheng, Rui Zhang, Yang Lu
Institution: Tianjin University of Science and Technology
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: hybrid
Estimated APC paid: $3,450
Award: G3941363345
OpenAlex ID: W4416091841