Work

Breaking the PAM Restriction: A Universal Double Stranded DNA Detection Method Based on the Sticky End-Mediated CRISPR/Cas12a Coupled RPA and Its Application to KRAS G12C Single Base Mutations

Year: 2025

Type: article

Source: Analytical Chemistry

DOI: 10.1021/acs.analchem.5c05908

Authors: Xinyu He, Liyuan Deng, Shiying Zhou, Tao Gu, Xinyao Li, Shuyu Zhu, Xiaogang Luo, Danqun Huo, Changjun Hou

Institution: Chongqing University

Language: English

Topic: CRISPR and Genetic Engineering

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: closed

Funders: National Natural Science Foundation of China, Graduate Scientific Research and Innovation Foundation of Chongqing, Fundamental Research Funds for the Central Universities

Awards: G1070951701, G2069037566, G6081912276

OpenAlex ID: W4416112763

FWCI: 1.015

Cites: 30

Cited by: 4

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