Work

Highly selective and cost-effective impedimetric biosensor for cylindrospermopsin detection using an aptamer-functionalized pencil graphite electrode

Year: 2025

Type: article

Abstract: Nutrient runoff from anthropogenic eutrophication, rising atmospheric CO 2 levels, and global warming have significantly contributed to the proliferation of harmful cyanobacterial blooms in freshwater ecosystems. These blooms release cyanotoxins, such as cylindrospermopsin ( CYN ), which pose genotoxic and cytotoxic effects. The detection and monitoring of CYN in water are essential for protecting public health and reducing environmental risks. In this study, an electrochemical aptasensor was developed by effective immobilization of CYN-specific aptamer ( cynApt ) onto a highly conductive pencil graphite electrode ( PGE ), which offers a sustainable alternative to expensive gold and graphene-based sensors. CYN was detected through electrochemical impedance spectroscopy ( EIS ) by monitoring changes in charge transfer resistance. The detection limits for CYN using PGE/cynApt electrodes were found to be 0.78 ng mL -1 in deionized water and 0.97 ng mL -1 in lake water, within a linear range of 1–20 ng mL -1 . The selectivity of the biosensor for CYN was also tested against other cyanotoxins, such as okadaic acid and saxitoxin, both in deionized and lake water.

Source: Toxicon

DOI: 10.1016/j.toxicon.2025.108657

Authors: Ece Kesici-Meco, Ece Unur-Yilmaz

Institution: Bursa Technical University

Language: English

Topic: Advanced biosensing and bioanalysis techniques

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Clean water and sanitation

Open Access status: hybrid

Estimated APC paid: $3,340