Work

Rab27a-mediated exosome secretion facilitates classical swine fever virus release and immune evasion

Year: 2025

Type: article

Abstract: family. Exosomes are extracellular vesicles that mediate intercellular communication by transferring membrane components and nucleic acids between cells. They play a role in the progression of various infectious diseases and help viruses evade immune responses. However, the impact of CSFV on exosome secretion and whether exosomes facilitate CSFV's evasion of the host immune response remains unclear. In this study, we first demonstrated that CSFV infection upregulates Rab27a, a small GTPase that regulates exosome secretion by mediating the trafficking and fusion of multivesicular bodies with the plasma membrane. By overexpressing and silencing Rab27a, we assessed its involvement in CSFV replication and release. We also demonstrated an interaction between the CSFV E0 and E2 proteins and Rab27a. Our findings show that Rab27a facilitates the release of exosomes, which, in turn, enhances CSFV spread. These results suggest that CSFV infection upregulates Rab27a, promoting the exosome-mediated release pathway, thereby contributing to viral spread and immune evasion. IMPORTANCE: family, is highly contagious. Exosomes, extracellular vesicles that transfer membrane components and nucleic acids, play a role in viral progression and immune evasion. This study shows that CSFV infection upregulates Rab27a, and the CSFV E0 and E2 proteins interact with Rab27a. By manipulating Rab27a expression, we found that Rab27a facilitates exosome release, which enhances CSFV spread. These findings suggest that CSFV exploits Rab27a to promote exosome-mediated release, aiding viral spread and immune evasion.

Source: Journal of Virology

DOI: 10.1128/jvi.01488-25