Year: 2025
Type: article
Abstract: Introduction: Antibodies and their derivatives constitute a crucial class of molecules in modern biotechnology and therapeutic development. Consequently, identifying chemically robust affinity ligands capable of specifically recognizing antibodies remains an important challenge. Methods: In this study, an in-house phage display library based on the WW domain scaffold (WWp5_4) was utilized to identify binders against polyclonal human IgG. A lead ligand from clone E6 was selected. To enhance the developability of WW ligands derived from this library, we rationally minimized structural liabilities within the scaffold framework to improve chemical stability and solubility, generating two mutant variants. Furthermore, a head-to-tail dimeric version of the E6 mutant sequence was designed. Results: The lead ligand E6 exhibited a dissociation constant of 133 nM. The mutant variants demonstrated a fivefold increase in expression yield compared to the native sequence. Additionally, the dimeric construct showed improved solubility and an estimated dissociation constant of 62 nM toward human IgG. Conclusions: This study underscores the potential of small folded protein domains, such as WW domains, as versatile affinity ligands for antibodies and other molecular targets, thereby broadening their applicability in biotechnology and bioengineering.
Language: English
Subfield: Radiology, Nuclear Medicine and Imaging
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $3,801
Awards: G1348429418, G1516346500, G1948874405, G2404034349, G2673245850, PROTEIOS - Protein Tunable Materials for Non-Invasive Sensing, Unidade de Ciências Biomoleculares Aplicadas, Unidade de Ciências Biomoleculares Aplicadas, G434337789, G547185811, G5506743709, G6205419041, Instituto para a Saúde e a Bioeconomia, Precisely Patterned Nanofibers for High Performance Bioseparations, G8839327758, G946021960, G949874453
OpenAlex ID: W4416128148