Year: 2025
Type: article
Abstract: Proteomics has demonstrated that each protein consists of various proteoforms that provide an additional layer of biological data next to gene-, transcript-, or protein expression levels. As a result, proteome analyses are increasingly being complemented with proteoform maps. Identification and quantification of the type, site, and dynamics of a proteoform modification contribute to a better understanding of human biology. Testing the hypothesis that proteoform-resolved data can provide novel tools in precision medicine requires robust and high-throughput proteoform measurements. The prime candidate for this purpose is top-down proteomics (TDP), commonly performed with mass spectrometry. In this Special Issue: Top-Down Proteomics, Fornelli et al. report a comparative analysis of various SDS-removal methods that are needed for proteoform mapping in TDP experiments. Their work provides technical guidance on an important aspect of the TDP sample preparation process.
Source: PROTEOMICS
DOI: 10.1002/pmic.70077
Author: Yuri E. M. van der Burgt
Institution: Leiden University Medical Center
Language: English
Subfield: Spectroscopy
Field: Chemistry
Domain: Physical Sciences
Open Access status: hybrid
Estimated APC paid: $4,650
OpenAlex ID: W4416129110