Work

Dietary Glycerol Monolaurate Effect on Growth Performance, Intestinal Barrier Function, and Gut Microbiota in Weaned Piglets Challenged by Lipopolysaccharide

Year: 2025

Type: article

Abstract: This study aimed to investigate the effects of glycerol monolaurate (GML) on the growth performance, intestinal morphology, inflammatory factors, and gut microbiota of weaned piglets challenged by lipopolysaccharide (LPS). A total of eighteen weaned piglets [Duroc × (Landrace × Yorkshire), average weight 7.54 ± 0.86 kg] were randomly assigned to the following three groups: (1) CON:basal diet; (2) LPS: basal diet + LPS challenge; and (3) LPS_GML: with 1000 mg/kg GML. On day 21, LPS or saline was injected into the piglets via intraperitoneal injection, and samples were collected after 4 h. Results showed that no differences were observed in the average daily gain (ADG), average daily feed consumption (ADFI), and G/F ratio. GML supplementation increased (p < 0.05) the villus height and villus height/crypt depth ratio, the protein expression of claudin-1 and occludin, the mRNA expression of claudin-1, SOD, and GSH-Px, while decreased (p < 0.05) the serum diamine oxidase (DAO) and reactive oxygen (ROS) concentrations and the mRNA expression of IL-1β, IL-6, and IL-8 compared with the LPS group. Moreover, GML regulated LPS-induced gut microbiota dysbiosis by reshaping the composition of gut microbiota, such as increasing (p < 0.05) Actinobacteriota, Lactobacillus amylovorus DSM 20531, Lactobacillus mucosae LM1, and Bifidobacterium boum, while decreasing (p < 0.05) Spirochaetota. Dietary GML supplementation improved the barrier function, reduced the inflammatory factors, and modulated the composition of gut microbiota in piglets challenged by LPS.

Source: Animals

DOI: 10.3390/ani15223263