Investigation of the Immune System Effects of Desloratadine on Lipopolysaccharide‐Stimulated Mammalian Macrophage Cells
Year: 2025
Type: article
Abstract: BACKGROUND: antihistamine, has been suggested to exert immunomodulatory effects, yet its impact on innate immune cells remains unclear. METHODS: J774.2 macrophages were treated with DES (1-10 μg/mL) ± lipopolysaccharide (LPS, 1 μg/mL). Cytokine secretion (IL-6, TNF-α, IL-12p40, GM-CSF) was measured by ELISA, and cell viability was assessed using Trypan Blue exclusion. RESULTS: DES induced a dose-dependent suppression of TNF-α, with significant inhibition at higher concentrations, and reduced IL-12p40 at 10 μg/mL. IL-6 showed a modest, nonsignificant decrease, whereas GM-CSF was increased, indicating a pro-inflammatory effect on macrophage activation. No cytotoxicity was detected at any dose. CONCLUSIONS: DES selectively modulates macrophage responses, strongly inhibiting TNF-α and IL-12p40 while enhancing GM-CSF, reflecting a dual anti- and pro-inflammatory profile. These findings support further investigation of DES in neuroinflammatory and immune-mediated conditions.
DOI: 10.1111/fcp.70056
Authors: Batuhan Yurtseven, Ömer Mete Başkan, Esra Aydemir, Furkan Ayaz
Institutions: Biruni University, Istinye University
Language: English
Topic: Mast cells and histamine
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: bronze
OpenAlex ID: W4416135727