Regulation of corneal stromal cell behavior by modulating curvature using a hydraulically-controlled organ chip array
Year: 2025
Type: article
Abstract: Corneal curvature abnormalities drive ectatic diseases, yet their mechanobiological effects on stromal cells remain poorly understood. We developed a hydraulically controlled curvature array chip recapitulating disease-relevant geometries (33-56D) to investigate how keratocytes, fibroblasts, and myofibroblasts respond to geometric stress. Curvature-induced mechanical stress triggered dramatic cellular remodeling keratocytes exhibited significant proliferative enhancement and phenotypic transformation with ALDH3A1 downregulation and α-SMA upregulation, indicating mechanobiologically driven fibrotic activation. Fibroblasts developed curvature-dependent orthogonal alignment that recapitulates native corneal lamellar organization without chemical cues, while myofibroblasts showed enhanced contractile responses. RNA sequencing revealed that geometric stress activates identical molecular pathways dysregulated in keratoconus, including TGF-β/SMAD signaling, ECM-receptor interactions, and inflammatory cascades. Extracellular matrix remodeling was cell-type specific, with keratocytes showing homeostatic control loss, fibroblasts promoting matrix deposition, and myofibroblasts driving degradation. These findings establish curvature-induced mechanotransduction as the fundamental driver of corneal ectatic disease progression, repositioning geometric stress from a passive consequence to an active determinant of pathology.
Source: Nature Communications
Authors: Minju Kim, Kanghoon Choi, David Križaj, Jungkyu Kim
Institution: University of Utah
Language: English
Subfield: Radiology, Nuclear Medicine and Imaging
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $6,990
Awards: G3406535317, G4060942575, G431582496, G4761588645, G5054609923, University of Utah Core Vision Research Grant, G5555614831, Cellular and Molecular Mechanisms that Contribute to Pressure-Induced Retinal Inflammation and Pathology, G5952210540, Utah Center for Clinical and Translational Science, Role of mechanosensation in retinal function and dysfunction, G869678598, G9000111304, G9000203035, CTSA UM1 Program at University of Utah, Vision Research Training Grant at the University of Utah
OpenAlex ID: W4416142909