Work

Multi-omics elucidation of resource allocation for enhanced ethanol production via precise glucose control in anaerobic Saccharomyces cerevisiae fermentation

Year: 2025

Type: article

Abstract: Ethanol, a high-demand clean energy source, is primarily produced via fed-batch fermentation in industrial settings. Although our previous study identified an optimal glucose concentration of 30 g/L for maximal ethanol yield, the mechanisms underlying glucose-dependent cellular adaptation remain unclear. Here, we performed an integrated multi-omics analysis, including transcriptomics, proteomics, metabolomics, and fluxomics, to compare yeast cells under glucose-controlled and uncontrolled conditions. Our results indicate that high glucose stress triggers the regulation of transporters with different affinities and the upregulation of heat shock proteins (HSPs), trehalose, and amino acids. In contrast, protein turnover was reduced under glucose-controlled conditions, suggesting more efficient resource allocation. This metabolic reallocation enhances carbon flux through glycolysis, potentially providing additional energy and NADH to support biomass growth and ethanol production. These findings advance our understanding of yeast regulatory mechanisms under glucose stress and provide insights for metabolic engineering and process optimization.

Source: Synthetic and Systems Biotechnology

DOI: 10.1016/j.synbio.2025.10.015

Authors: Zhihao Liu, Linghong Zheng, Xiaofei Yu, Yingping Zhuang, Guan Wang

Institutions: Chinese Academy of Sciences, Qingdao Institute of Bioenergy and Bioprocess Technology, East China University of Science and Technology, State Key Laboratory of Bioreactor Engineering

Language: English

Topic: Microbial Metabolic Engineering and Bioproduction

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Affordable and clean energy

Open Access status: gold

Estimated APC paid: $1,500