The protein-protein interaction between two spliced leader trans-splicing factors is mediated by two interlinked α-helical domains.
Year: 2025
Type: article
Abstract: . They are components of the SL1 snRNP that donates the spliced leader 1 RNA which replaces the 5' end of most pre-mRNAs. SNA-1 and SNA-2 bind to each other, but the nature of their interaction is unclear. AlphaFold-Multimer predicts that the central region of SNA-1 and the C-terminal region of SNA-2 , each consisting of 3 α-helices, interlink to bring about the interaction between these proteins. Using yeast 2-hybrid assays we demonstrate that these regions are required and sufficient for this unusual mode of interaction between two proteins.
Source: PubMed
Authors: Peter Eijlers, Jonathan Pettitt, Berndt Müller
Institution: University of Aberdeen
Language: English
Topic: RNA Research and Splicing
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W4416168573
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