Combining Nanoscale Curvature and Polymer Osmotic Pressure for Efficient Giant Vesicle Assembly Under Physiological Conditions
Year: 2025
Type: article
Abstract: Thin film hydration methods are widely used to assemble giant unilamellar vesicles (GUVs), but their efficiency drops sharply in solutions of physiological ionic strength due to enhanced adhesion between stacked lipid bilayers, which suppresses bud and foam-like mesophase formation. Here, polymer-coated nanocellulose paper (PCP) is introduced as a nanostructured substrate that overcomes this limitation. Hydration of lipid films on PCP, termed PCP-assisted hydration, achieves high GUV yields across a broad temperature range (4 °C-45 °C) using diverse soluble polymers, including ultralow-gelling agarose, hyaluronic acid, dextran, carrageenan, and polylysine. The nanoscale curvature of the cellulose fibers and the osmotic pressure generated by polymer dissolution act synergistically to promote membrane budding even under physiological salt conditions. The approach is scalable-supporting GUV production from millimeter-sized pieces to large-format sheets-and biocompatible, enabling encapsulation of complex biochemical systems such as cell-free expression mixtures and actin-fascin assemblies. PCP-assisted hydration thus provides a robust, versatile, and high-yielding platform for generating functional GUVs under physiological conditions.
Source: Small Methods
Institution: University of California, Merced
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $4,640
Awards: MRI: Acquisition of an Upright Laser Scanning Confocal Microscope to Advance Research and Education at the University of California, Merced, G2044507624, G2892984557, G3148101318, G3504179459, G3860033866, WE PROPOSE TO ESTABLISH A RESEARCH AND EDUCATION CENTER OF EXCELLENCE MACES BY HARNESSING THE STRONG NANOMATERIAL BASED RESEARCH PROGRAMS THAT EACH P, CREST Center for Cellular and Biomolecular Machines, G4631170063, CREST Center for Cellular and Biomolecular Machines, G5433188078, G5613623313, CAREER: Understanding the fundamental mechanisms of vesiculation and solute encapsulation of smectic phospholipid films on cellulose, G6671297155, G8051978230
OpenAlex ID: W4416186509