Work

Continuous scanning full-field OCT for fast volumetric imaging of multi-cellular aggregates

Year: 2025

Type: article

Abstract: Full-field optical coherence tomography (FF-OCT) offers label-free, high-resolution imaging of biological samples but remains limited by slow acquisition due to piezoelectric mirror modulation. We present a continuous-scanning FF-OCT method that eliminates piezoelectric displacement and synchronization by continuously translating the sample with a motorized stage while recording images on the fly. Depth-resolved information is retrieved via Fourier analysis of the temporal signal at each pixel. This approach enables volumetric imaging over several hundred micrometers within tens of seconds and provides a higher contrast-to-noise ratio than traditional four-phase FF-OCT. Continuous-scanning FF-OCT thus represents a simpler and faster alternative for 3D bio-imaging of living tissues and organoids.

Source: Biomedical Optics Express

DOI: 10.1364/boe.578177

Authors: C. Kersuzan, A. Jana, A. Badon

Institutions: Centre National de la Recherche Scientifique, Université de Bordeaux, Laboratoire Photonique, Numérique et Nanosciences

Language: English

Topic: Optical Coherence Tomography Applications

Subfield: Biomedical Engineering

Field: Engineering

Domain: Physical Sciences

Open Access status: gold

Estimated APC paid: $1,913

Funders: Agence Nationale de la Recherche, Université de Bordeaux