Supplementary Figure S3 from Evolution and Co-occurrence of PI3K Pathway Gene Mutations in Endometrial Carcinoma Molecular Subtypes at the Single-Cell Level
Year: 2025
Type: supplementary-materials
Abstract: Supplementary Figure S3. Somatic mutations present in all endometrioid endometrial cancers included in this study, correlation between variant allele fractions of the mutations detected both in single-nucleus and matched bulk sequencing data, and immunohistochemical analysis of the mismatch repair genes. A, Heatmap depicting all of the somatic mutations detected both in single-nucleus and matched bulk sequencing in endometrioid endometrial cancers. Samples are shown in columns and variants in rows. The alteration types are color coded according to the legend. B, Agreement of the allele-fraction (AF) of each variant determined by our single-nucleus sequencing assay (y-axis) and matched bulk high-depth targeted sequencing data (x-axis) (Pearson correlation), per molecular subtype. C, Representative micrographs of the MLH1, PMS2, MSH2 and MSH6 immunohistochemical analysis of the mismatch repair deficient EEC87 (top left), EEC128 (top right) and EEC133 (middle left) tumor samples, and of the POLE mutant EEC14 (middle right) and EEC131 (bottom left) tumor samples. Heatmap depicting the genetic somatic alterations affecting the mismatch repair genes (bottom right). Samples are shown in columns and genes in rows. The alteration types are color-coded according to the legend. EEC, endometrioid endometrial carcinoma.
Language: English
Subfield: Obstetrics and Gynecology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4416195757