Quantitative real-time PCR detection of Porphyromonas gingivalis and Filifactor alocis in peri-implantitis
Year: 2025
Type: article
Study design: Observational Study
Abstract: Aim: To assess the prevalence and levels of P. gingivalis and F. alocis in peri-implantitis and healthy peri-implant sites using quantitative real-time PCR (qPCR).Materials and Methods: This cross-sectional study included 110 participants, 52 with peri-implantitis and 58 with healthy peri-implant sites. Clinical parameters were recorded, including probing depth, clinical attachment level, and bleeding on probing. Microbiological samples were analyzed using qPCR, and significance was tested using the Mann-Whitney U test and Spearman’s rank correlation.Results: P. gingivalis and F. alocis levels were significantly higher in peri-implantitis sites, with P. gingivalis at 4.80 × 10⁶ ± 4.78 × 10⁶ copies/µL and F. alocis at 4.58 × 10⁵ ± 3.40 × 10⁵ copies/µL, compared to healthy sites, with P. gingivalis at 2.09 × 10³ ± 1.26 × 10³ copies/µL and F. alocis at 2.45 × 10³ ± 1.64 × 10³ copies/µL, with p < 0.001. P. gingivalis strongly correlated with clinical parameters, such as probing depth, clinical attachment level, and bleeding on probing. F. alocis showed moderate correlations with probing depth and clinical attachment level but not bleeding on probing.Conclusion: The findings suggest a potential synergistic role of P. gingivalis and F. alocis in peri-implantitis, emphasizing the importance of therapies targeting these pathogens. Clinicians might explore antimicrobial strategies disrupting biofilm formation and microbial synergy to improve outcomes. Further research is needed to refine treatment approaches and understand these bacteria's contributions to disease progression.
Source: Journal of Medical Microbiology
DOI: 10.1099/jmm.0.002091
Institution: Aristotle University of Thessaloniki
Language: English
Subfield: Periodontics
Field: Dentistry
Domain: Health Sciences
Open Access status: hybrid
OpenAlex ID: W4416203517