Tenacibaculum salmonis sp. nov., isolated from Atlantic salmon (Salmo salar L.) fish farmed in Chile
Year: 2025
Type: article
Abstract: Strain P3-BQ1 T is a Gram-negative, aerobic, non-spore-forming bacterium with gliding motility and filamentous cells. It was isolated in 2018 from the gills of a diseased Atlantic salmon ( Salmo salar ) during an outbreak of tenacibaculosis in a Chilean fish farm. Phylogenetic analysis based on 16S rRNA gene sequencing confirmed that strain P3-BQ1 T belongs to the genus Tenacibaculum and is most closely related to Tenacibaculum dicentrarchi 35/09 T (98.53%), ‘ Tenacibaculum pacificus ’ 18-2881-A (98.46%), Tenacibaculum aestuariivivum JDTF-79 T (98.46%), Tenacibaculum finnmarkense TNO010 (97.85%) and Tenacibaculum piscium TNO20 T (97.41%). The genome size of strain P3-BQ1 T is 2,777,603 bp, with a DNA G+C content of 29.18 mol%. The strain has an OrthoANI score lower than 95% compared to the types of all validly named Tenacibaculum species, with the closest neighbour being T. dicentrarchi 35/09 T (score 87.41 %). In contrast, P3-BQ1ᵀ and ‘ T. pacificus ’ 18-2881-A shared a 96.58%, thus exceeding the species threshold. This result is also supported by whole-genome in silico DNA–DNA hybridization/Genome-to-Genome Distance Calculator values, indicating that strains P3-BQ1 T and 18-2881-A are conspecific. Strain P3-BQ1 T contains MK-6 as its sole detectable menaquinone. The polar lipids profile includes glycolipids ( n =2), aminolipids ( n =3) and unidentified lipids ( n =5). The predominant cellular fatty acids (>5 %) are C 13 : 1 , C 15 : 0 , iso-C 15 : 0 , iso-C 15 : 0 3-OH and summed feature 3 (C 16 : 1 ω7с/C 16 : 1 ω6с). An immersion challenge was conducted to assess the pathogenic potential in Atlantic salmon. Our findings indicate that strain P3-BQ1 T alone does not cause significant mortality, suggesting it is likely non-pathogenic to this species. Based on the phenotypic, phylogenetic and genotypic data presented, Tenacibaculum salmonis sp. nov. is proposed here with type strain P3-BQ1 T (=IMI 507635 T =RGM 3581 T ), being 18-2881-A an additional strain.
Authors: Ruben Avendaño-Herrera, Rute Irgang, Pierre Lopez
Language: English
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
SDG: Life below water
Open Access status: hybrid
Awards: G1063360175, G4885317604, G4909873887, ANTICIPATING INSTEAD OF REACTING TO THE IMMINENT THREAT OF BACTERIAL COINFECTIONS IN FARMED SALMON ELUCIDATION OF FISH SUSCEPTIBILITY AND TREATMENT EFFICACIES DURING COINFECTION IN ATLANTIC SALMON WITH CAUSATIVE ISOLATES OF BKD AND/OR TENACIBACULOSIS., INTERDISCIPLINARY CENTER FOR AQUACULTURE RESEARCH (INCAR)
OpenAlex ID: W4416217955