Work

Epi Multiome ATAC + Gene Expression – Stanford Bone Marrow TMC v1

Year: 2025

Type: preprint

Abstract: Fresh human whole bone marrow aspirates (25 mL; AllCells) were collected and processed on the same day. Mononuclear cells were isolated by Ficoll density gradient centrifugation followed by red blood cell lysis. CD34⁺ hematopoietic stem and progenitor cells were enriched by magnetic selection (STEMCELL Technologies, catalog # 17856). Equal numbers of CD34⁺ and CD34⁻ cells (5×10⁵ each) were then pooled at a 1:1 ratio. Nuclei were extracted following the 10x Genomics Single Cell Multiome nuclei isolation protocol (see links below), generating high-quality nuclei suitable for downstream ATAC + gene expression multimodal profiling.

DOI: 10.17504/protocols.io.j8nlkyjw5g5r/v1

Authors: Patricia Favaro, Jumana Afaghani, Sean Bendall

Institution: Stanford University

Language: English

Topic: Single-cell and spatial transcriptomics

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

Funders: National Institutes of Health, National Heart, Lung, and Blood Institute

Award: Multimodal histologic atlas of human bone marrow