Year: 2025
Type: article
Abstract: BACKGROUND: The presence and function of microtubules within the synaptic bouton has long been under investigation. In recent years, evidence has accumulated that connects the synaptic vesicle cluster to the local dynamics of microtubule ends. Nonetheless, one question remains open, namely whether the vesicles influence the availability of tubulin within the synaptic compartment. RESULTS: An analysis of previously published live imaging experiments indicates that tubulin is strongly enriched in the synaptic vesicle cluster. To analyze the vesicle-tubulin interaction directly, we isolated vesicles from the mouse brain and imaged them together with fluorescent tubulin in vitro. We found that soluble tubulin is collected by synaptic vesicles in physiological buffers, resulting in the formation of tubulin-rich regions (TRRs) on the respective vesicle clusters. CONCLUSIONS: We conclude that the synaptic vesicle cluster is indeed able to recruit soluble tubulin.
Source: BMC Biology
Authors: Tiago Mimoso, Aleksandr Korobeinikov, Alexander Stein, Dragomir Milovanovic, Silvio O. Rizzoli, Sarah Köster, Sofiia Reshetniak
Institutions: University of Göttingen, German Center for Neurodegenerative Diseases, Max Planck Institute for Multidisciplinary Sciences, Humboldt-Universität zu Berlin, Einstein Center for Neurosciences Berlin, Freie Universität Berlin, Charité - Universitätsmedizin Berlin, Universitätsmedizin Göttingen, Multiscale Bioimaging
Language: English
Subfield: Cell Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $3,690
OpenAlex ID: W4416223928