Supplementary Figure S1 from Evolution and Co-occurrence of PI3K Pathway Gene Mutations in Endometrial Carcinoma Molecular Subtypes at the Single-Cell Level
Year: 2025
Type: supplementary-materials
Abstract: Supplementary Figure S1. Validation of single-nucleus sequencing method using cell line admixtures, single-nuclei isolation assessment and calculation of the site-specific background noise. A, Schematic representation of the HEC6 and Ishikawa endometrial carcinoma cell line mixtures (in a 1:10 ratio). Both cell line mixtures and respective parental cell lines were submitted to bulk targeted sequencing and single-cell DNA sequencing. B, Single nuclei preparation from frozen tissue is enriched for intact nuclei: representative confocal micrographs of DAPI-stained frozen nuclei. C, Calculation of the site-specific background noise as the mean posterior estimate of alternate allele depth using a Hierarchical Bayesian model. The icons in Supplementary Figure S1A were created by Biorender.com.
Language: English
Subfield: Obstetrics and Gynecology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4416232151