Work

Suppression of large t antigen-stimulated CD4+CD25+ and CD8+IFN-γ+ T cells is strongly associated with low level BK viremia in kidney transplant recipients

Year: 2025

Type: article

Abstract: Background: BK polyomavirus (BKPyV) infection following kidney transplantation results from over-suppression of cellular immunity. Currently, there is no established, clinically applicable immunological assay that comprehensively monitors cellular immune responses against BKPyV, incorporating both cytokine production and T cell activation markers. Our study aimed to comprehensively assess both cytokine production and surface activation markers to differentiate kidney transplant recipients (KTR) with low-level (<3,000 copies/mL) BKPyV viremia from those without viremia. Methods: Thirty-six participants were enrolled, comprising KTR with (BK) and without BKPyV viremia (nBK), alongside healthy controls (HC). Peripheral blood mononuclear cells (PBMC) were stimulated using BKPyV viral capsid protein-1 (VP1) or large-T-antigen (LTA), with and without CD28/CD49d co-stimulatory antibodies. Outcomes included expression of IL-2, IFN-γ, TNF-α, CD25, CD134, CD137, and CD154. Candidate markers were evaluated by calculating the area under the receiver operating characteristic curve (AUROC) for diagnosing BKPyV viremia. Results: = 0.028, respectively) at a cutoff of > 0.2% positive cells. Conclusion: T cells differentiated KTR with and without low-level BKPyV viremia, representing promising markers for early clinical diagnostics and future studies.

Source: Frontiers in Medicine

DOI: 10.3389/fmed.2025.1662833