In vivo microdialysis for determination of nasal liquid ion composition
Year: 2025
Type: article
Abstract: Airway surface liquid (ASL) contains substances important in mucociliary clearance and airway defense. Little is known about substance concentrations in ASL because of its small volume and sampling difficulties. We used in vivo microdialysis (IVMD) to sample liquid lining the nasal cavity without net volume removal and incorporated into IVMD a potential difference (PD) electrode to assess airway integrity. The cystic fibrosis (CF) mouse nasal epithelia exhibit ion transport defects identical to those in CF human airways and, thus, are a good model for CF disease. We determined that nasal liquid [Na+] (107 ± 4 mM normal; 111 ± 9 mM CF) and [Cl-1] (120 ± 6 mM normal; 122 ± 4 mM CF) did not differ between genotypes. The nasal liquid [K+] (8.7 ± 0.4 mM) was significantly less in normal than in CF mice (16.6 ± 4 mM). IVMD accurately samples nasal liquid for ionic composition. The ionic composition of nasal liquid in the normal and CF mice is similar.
DOI: 10.17615/4qyv-3r86
Authors: Chadburn J.L., Boucher R.C., Grubb B.R.
Institution: University of North Carolina at Chapel Hill
Language: English
Subfield: Pulmonary and Respiratory Medicine
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W4416236903
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