Work

Role of claudin interactions in airway tight junctional permeability

Year: 2025

Type: article

Abstract: Airway epithelial tight junctions (TJs) serve to separate the external and internal environments of the lung. However, the members of the claudin family that mediate this function have not been fully delineated. We characterized the claudin expression in normal airways removed from human donors during lung transplantation and determined the contribution of each claudin to airway barrier function. Stable cell lines in NIH/3T3 and human airway (IB3.1) cells were constructed expressing the claudin components found in the human airway, claudin-1, -3, or -5. The effects of claudin expression on transepithelial resistance, permeability coefficients, and claudin-claudin interactions were assessed. Claudin-1 and -3 decreased solute permeability, whereas claudin-5 increased permeability. We also detected oligomerization of claudin-5 in cell lines and in freshly excised human airways. Coimmunoprecipitation studies revealed heterophilic interactions between claudin species in both cell lines and human airway epithelium. These suggest that airway TJs are regulated by claudin-claudin interactions that confer the selectivity of the junction.

Source: Carolina Digital Repository (University of North Carolina at Chapel Hill)

DOI: 10.17615/spbv-vm16

Authors: Coyne C.B., Johnson L.G., Carson J.L., Gambling T.M., Boucher R.C.

Institution: University of North Carolina at Chapel Hill

Language: English

Topic: Barrier Structure and Function Studies

Subfield: Neurology

Field: Neuroscience

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

OpenAlex ID: W4416236904

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