Year: 2025
Type: article
Abstract: H nuclear magnetic resonance (NMR) spectroscopy. Given previous observations of elevated reactive species under glucose-free conditions, we explored the cellular adaptations associated with a metabolic shift from glycolysis to oxidative phosphorylation (OXPHOS). Cells cultured in OXm exhibited increased metabolic activity, elevated protein content, and substantial metabolic remodeling. Morphological analysis revealed enlargement of the cell body, cytoplasm, mitochondria, and nuclei, indicative of extensive structural adaptation. Notably, oxygen consumption rate (OCR) nearly doubled within 24 h of exposure to OXm, reflecting a rapid mitochondrial response to metabolic stress. The presence of the antioxidant N-acetyl cysteine (NAC) attenuated this increase, suggesting that redox signaling plays a key role in mitochondrial bioenergetic adaptation. These findings underscore the complex interplay between metabolic context, oxidative stress, and cellular morphology, and highlight the importance of appropriate normalization strategies in metabolic studies. See also the graphical abstract(Fig. 1).
Source: EXCLI journal
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: diamond
Awards: G1167214205, G1483381719, Centro de Inovação em Biomedicina e Biotecnologia, G2120309575, G2476410813, DE-RISKING METABOLIC, ENVIRONMENTAL AND BEHAVIORAL DETERMINANTS OF OBESITY IN CHILDREN, ADOLESCENTS AND YOUNG ADULTS, G4015828041, G4705738886, G491858407, G5642561709, G6219476557, G6255773888, Centro de Inovação em Biomedicina e Biotecnologia, G6886798228, Centro de Inovação em Biomedicina e Biotecnologia - Laboratório Associado, MitoInformatics: Computational Tools for the Assessment of MitochodrianHealth, G810402943
OpenAlex ID: W4416239128
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