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Determination and pharmacokinetic study of thyroid hormone receptor-β agonist resmetirom in beagle dogs using HPLC-MS/MS method

Year: 2025

Type: article

Abstract: A HPLC-MS/MS method for determination of thyroid hormone receptor-β agonist resmetirom was established and validated, and pharmacokinetic study was conducted in beagle dogs after oral administration. Methanol-0.1% formic acid was used as the mobile phase, resmetirom and carbamazepine (internal standard, IS) were separated on a VP-ODS C18 column. During the MS detection process, multiple reaction monitoring (MRM) in electrospray positive ion mode was used. The quantitative ions were m/z 435.10→182.05 for resmetirom, and m/z 237.30→194.30 for IS. The linear range of resmetirom was 1-200 ng/mL, with correlation coefficients greater than 0.999 8. The precision RSD values of resmetirom were 3.14~8.42% and 2.74~7.87%, respectively. The accuracy values were around 100%. The recovery of resmetirom were greater than 80.39%. At the same time, resmetirom were stable under the experimental conditions. After oral administration of resmetirom to the beagle dogs, the C max of resmetirom was about 802.40 ng/mL at about 3.33 h, the t 1/2 of resmetirom was about 4.58 h.

Source: Journal of Pharmaceutical and Biomedical Analysis

DOI: 10.1016/j.jpba.2025.117249

Authors: Songmao Liang, Mingxuan Li, Xu Gong, Zhe Luo, Jiman Yang

Institution: Henan University of Science and Technology

Language: English

Topic: Analytical Methods in Pharmaceuticals

Subfield: Analytical Chemistry

Field: Chemistry

Domain: Physical Sciences

SDG: Good health and well-being

Open Access status: hybrid

Estimated APC paid: $3,680