Year: 2025
Type: article
Abstract: . The discovery relied upon a metabolic glycan labeling strategy in combination with commonly used phase-separation-based RNA isolation. Following the reported procedure, here we likewise identify an N-glycosylated species in the RNA fraction. However, our results suggest that the reported RNase sensitivity of the glycosylated species depends on the specific RNA purification method. This suggests the possibility of copurifying unexpected RNase-insensitive N-glycoconjugates during glycoRNA isolation. The co-existence of two independent, yet highly similar molecular entities, complicates biochemical assays on glycoRNA and calls for more specific approaches for glycoRNA analysis. To address this, we propose a control experiment that can help distinguish genuine glycoRNA species from copurified glycoconjugates.
Institutions: Pohang University of Science and Technology, Yonsei University, Institute for Basic Science, Utrecht University, Radboud University Medical Center, Radboud University Nijmegen, Whitehead Institute for Biomedical Research, Delft University of Technology, University of Wrocław, Leiden University, Leiden University Medical Center, University Medical Center Utrecht, Oncode Institute, Hubrecht Institute for Developmental Biology and Stem Cell Research, Ewha Womans University
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $4,490
Awards: G1839150630, G2351161035, G2402660949, G2643796712, G361741885, G3744481246, Repurposing small RNA from ciliates for genome editing: single-molecule study, G6128294776, Cell Surface RNAs as Therapeutic Targets, The sweet touch – glycoRNAs as novel players in immune cell communication, G8739444332
OpenAlex ID: W4416247932