Work

COX-2 gene silencing by laser-assisted oligonucleotides release from remote optical Nano-switches (LORONS) in hepatocellular carcinoma cells

Year: 2025

Type: article

Abstract: Background Overexpression of cyclooxygenase-2 (COX-2) has been associated with hepatocellular carcinoma (HCC). Selective inhibition of COX-2 can come forward as improved and targeted therapeutic strategy.Methods We hereby determine selective inhibition efficiency of COX-2 enzyme in HepG2 cells through laser-assisted oligonucleotides release from remote optical nano-switches (LORONS) with spatial and temporal control. Gold nanorods (GNRs) were decorated with fluorescein labeled single and double strand RNA interfering oligos through methoxy PEG thiol linkages. Upon uptake by HepG2 cell, conjugated GNRs were exposed to continuous NIR laser irradiation near the resonance wavelength of GNRs (808 nm) for controlled release of oligos.Results COX-2 protein expression was reduced by 93% after NIR laser exposure compared to control sample after 48 h (p < 0.05). Significant reduction in prostaglandin E2(PGE2) levels after LORONS treatment was also observed. Gene silencing efficacy using GNRs conjugated oligos without laser exposure was recorded 38%.Conclusion We hereby conclude LORONS as a useful therapeutic strategy for localized gene silencing by remote optical excitation at desired intracellular location.

Source: Nanomedicine

DOI: 10.1080/17435889.2025.2584581

Authors: Uzma Azeem Awan, Abida Raza, Shaukat Ali, Mostafa El Sayed

Institutions: Georgia Institute of Technology, National University of Medical Sciences, Pir Mehr Ali Shah Arid Agriculture University, Government College University, Lahore

Language: English

Topic: Nanoplatforms for cancer theranostics

Subfield: Biomedical Engineering

Field: Engineering

Domain: Physical Sciences

SDG: Good health and well-being

Open Access status: green

Funder: Pakistan Science Foundation