Year: 2025
Type: article
Abstract: Transcription in the cell is carried out by specialized enzymes - RNA polymerases. RNA polymerases transcribe DNA with the formation of elongation complexes (EC), which have a regulatory significance. Cryo-electron microscopy (cryo-EM) allows obtaining the structures of these complexes and clarifying the mechanisms of transcription stages. However, the preparation of EC samples suitable for cryo-EM studies presents certain challenges. In this work, a selection of protocols for the preparation of EC+39 samples was carried out. The formation of complexes is confirmed by the results of electrophoresis and negative stain electron microscopy. The results obtained can be used to study EC+39 by the cryo-EM method.
Authors: E.V. Osina, A.V. Moiseenko, A.N. Korovina, N.S. Gerasimova, O.I. Volokh, V.M. Studitsky, T.B. Stanishneva-Konovalova
Institutions: Lomonosov Moscow State University, Fox Chase Cancer Center
Language: Russian
Subfield: Structural Biology
Domain: Life Sciences
Open Access status: hybrid
Funder: Russian Science Foundation
OpenAlex ID: W4416308946