Whole-genome analysis of qacA-harboring ST45-SCCmec Vc-t1081 MRSA from skin exudate in a Chinese patient with severe drug eruption
Year: 2025
Type: article
Abstract: The methicillin-resistant Staphylococcus aureus (MRSA) sequence type (ST) 45 is a significant global lineage of MRSA, known for its vast strain diversity and substantial clinical impact. This study aimed to characterize the genomic characteristics and diversity of the ST45-SCC mec Vc-t1081 MRSA strains. MRSA strain SA0907 was isolated from the skin exudate of a patient with severe drug eruption in China. The complete genome of MSRA strain SA0907 was sequenced using both Illumina (Novaseq 6000) and PacBio (Sequel II) platforms, followed by hybrid assembly of the sequences. The identification of mobile genetic elements and antibiotic resistance genes was accomplished through bioinformatics tools. The SCC mec type Vc in SA0907 was comprised of the class C2 mec gene complex (IS 431 - mecA -Δ mecR1 -IS 431 ) and type 5 ccr gene complex ( ccrC1 allele 2 and ccrC1 allele 8). Notably, the MRSA strain SA0907 was the first complete genome data for the ST45-SCC me c Vc-t1081 MRSA in Mainland of China. The disinfectant resistance genes qacA / qacR were chromosomally located within a variable region containing Tn 552 and Tn 4001 in MRSA strain SA0907. The 2.46-kb plasmid pSA0907-2 from MRSA strain SA0907 harbored erm(C) , which conferred MLS B (macrolides-lincosamides-streptogramins B) resistance. Small plasmids similar to the 2.46-kb plasmid pSA0907-2 were prevalent across the Staphylococcus genus, particularly in S. aureus . MRSA strain SA0907 represents the first complete genome report of a qacA -harboring ST45-SCC mec Vc-t1081 MRSA in Mainland of China. One 2.46-kb erm(C) -harboring plasmid pSA0907-2 carried by the MRSA strain SA0907 was widely present in S. aureus and other staphylococci.
Authors: Wei Kong, Qinghuan Zhang, Limei Zhang, Jing Yang, Xiaobin Li, Yanyan Liao
Language: English
Subfield: Infectious Diseases
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $1,890
OpenAlex ID: W4416356114