Work

The study on the involvement of exosomes in autophagy and apoptosis of vascular endothelial cells induced by HMME-PDT

Year: 2025

Type: article

Abstract: Significance This study investigated the mechanism of exosomes involved in HMME-PDT induced autophagy and apoptosis. Approach To investigate the mechanism of exosomes affecting autophagy and apoptosis of vascular endothelial cells, HUVEC group, HMME-PDT group, HMME-PDT+3-MA group and HMME-PDT+zVAD-fmk group were set up in this study. The secretion levels of hemoporfin from different groups of exosomes were analyzed. Next, human umbilical vein endothelial cells were treated with exosomes from different groups, and treated with 630nm laser irradiation to analyze and compare the differences in cell growth activity, apoptosis level, and autophagy level. Results HMME-PDT can induce HUVECs to release exosomes rich in hemoporfin. After inhibition of autophagy, hemoporfin concentration in exosomes is significantly decreased, while after inhibition of apoptosis, hemoporfin concentration in exosomes is significantly increased. The function verification experiments of exosomes further proved that exosomes can affect cell growth, apoptosis and autophagy. Conclusions Exosomes affect autophagy and apoptosis of HUVECs by mediating the emission of hemoporfin.

Source: Photodiagnosis and Photodynamic Therapy

DOI: 10.1016/j.pdpdt.2025.105044

Authors: Jingwen Xue, Yi Zhao

Institution: Beijing Tsinghua Chang Gung Hospital

Language: English

Topic: Photodynamic Therapy Research Studies

Subfield: Pulmonary and Respiratory Medicine

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $2,610